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  •   Gas Chromatography

 

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Gas Chromatography (GC) is an instrumental technique use to analyze organic compounds. The compounds with sufficient volatility and thermal stability could be analyzed using GC. The samples that come in different matrices such as water, soil, fertilizer, etc are extracted into organic solvents, separated from interfering substances and concentrated before introducing to the instrument. Once the sample is injected into the instrument it enters to the separation column with the carrier gas. Inside the column the compound gets separated into various components. The detector measures the components that exit from the column. Different detectors will give different types of selectivity.

 

 

In our lab we use GC with various detectors to conduct analysis of volatile organic and chlorinated organic compounds;

 

GC – FID/ECD   - Chlorinated organic compound/volatile organic compoundanalysis         

GC-ECD/NPD    - Chlorinated organic compound analysis

GCMS                - Volatile organic compound analysis

GCMS-MS         - Volatile organic compound analysis

 

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  •   High Performance Liquid Chromatography
hplc   

HPLC is an instrumental analytical technique to separate compounds into their components. Unlike in normal liquid chromatography where the liquid passes through the column under gravity, HPLC uses high pressure to pass the sample mixture through the column packed with a solid adsorbent material. As in other chromatographic techniques, separation of the sample into its constituents occurs according to their affinity to the mobile and stationary phase. The eluting components that come out from the column is detected by detectors. The detectors

At ITI, we use three types of HPLC in our analytical work.

HPLC- DAD   – Pesticides, food toxins, pharmaceuticals analysis

HPLC- FLD    - Pesticides, food toxins, pharmaceuticals analysis

HPLC- RID     - Pesticides, food toxins, pharmaceuticals analysis

 

 

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  •   LCMS-MS (Liquid Chromatograph- Tandem Mass Spectrometer (QTRAP)

     

    LCMSMS     

    LCMSMS (QTRAP) is one of the recently added high end instruments to Residue Alanysis Lab. LCMS-MS is usually a high-performance liquid chromatograph combined with two mass spectrometers. Here the injected sample which gets separated into different components in the LC column is directed into the first mass spectrometer where it is ionized. The resulting ions are then separated and detected in the second mass analyzer. The results of LSMS-MS in more accurate and précised with better selectivity.

    LCMS-MS is highly used in analysis of polar organic compounds in our lab.

     

     

  • Atomic Absorption Spectrophotometry

 

aas   

Atomic Absorption Spectrophotometeris a scientific instrument used to detect metals in solutions. Here the sample is fragmented into small metal atoms using anatomizer. These metal atoms interact with radiation of a pre-set wavelength which can be measured and interpreted. AAS can determine metals in parts per million to parts per billion concentration ranges with speed and affordable cost.

AAS-VGA       - Element Analysis

AAS-GTA       - Element Analysis

 

 

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  •   Inductively Coupled Plasma Mass Spectrometer

ICPMS

ICPMS is another latest addition to our Residual Analysis Laboratory. It is also an analytical technique used for elemental analysis. ICPMS is a type of mass spectrometer which is combined with inductively coupled Plasma. This instrument has the capability to detect metals at concentrations as low as one part in 1015

 

 

 

 

  • GR Microwave Extraction System

Neos-Neos-GR-and-MAC-75-Milestone-Technologies

 

The NEOS-GR is based on the MHG technology (Microwave Hydrodiffusion and Gravity) a new and green technique for the extraction of essential oils from different kinds of aromatic plants.

This green extraction is an original “upside down” microwave alembic combining microwave heating and earth gravity at atmospheric pressure. Based on a relatively simple principle, this method involved placing plant material in a microwave reactor, without adding any solvent or water.

It is important to note that this green method allows to extract essential oils without distillation and evaporation which are the most energy consuming between the unit operations. The distillation step which can represent up to 80% of the total analytical procedure is no longer required. Whereas traditional processes are carried out within 3 hours, solvent-free microwaves hydrodiffusion plus gravity requires no more than 30 minutes!
Once the essential oils have been extracted they can be analyzed directly by GC-MS without any preliminary clean-up or solvent exchange steps.

 

Application

 

  • Extraction of essential oils from medicinal plants/spices
  • Extraction of natural flavor compounds
  • Extraction of pigments, aroma components, antioxidants and other secondary metabolites from fruits/vegetables/medicinal plants

 

Contact Details: Herbal Technology Section

                             +94-11-2797322

                                                      

 

  • Research Microscope ECLIPSE Ts2R
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The ECLIPSE Ts2R sets a new standard for the inverted research microscope, providing a wide range of observation methods and applications in a compact body that can easily fit in limited laboratory spaces while maintaining excellent operability.

This new contrasting technique is compatible with both plastic and glass culture dishes. Unlike phase contrast or NAMC, Emboss Contrast does not require special objective lenses and therefore has minimal effect on Epi-fluorescence observation. Emboss Contrast allows thick samples such as embryos to be easily observed in pseudo-three-dimensional image with great clarity.

 

Contact Details: Herbal Technology Section

                            +94-11-2797322

 

  • CO2 Incubator
Incubater   

The MCO-20AIC CO2 Incubator delivers precise humidity/CO2 and temperature control for cell culture in medical, pharmaceutical, and medical research and clinical labs. This incubator has an interior capacity of 195 L with temperatures up to 50oC and up to 20% CO2 control. Features Safe Cell UV contamination control system, infared sensor CO2 control, and microprocessor PID control.

 

Contact Details: Herbal Technology Section

                            +94-11-2797322

 

  • uv / vis microplate reader - spectramax plus 384
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This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction.

  • Temperature control
  • Shaking capabilities
  • Multichannel optical design
  • Eliminates error

The patented multichannel optical design of the SpectraMax Plus384 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measuring each sample in the plate directly, eliminating measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting.

The nine-channel system delivers both high precision and speed when reading cuvettes, 96-well, and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including ELISAs in the visible range or DNA quantitation in the UV range.

Contact Details: Herbal Technology Section

                            +94-11-2797322

 

  • Gas chromatography-mass spectrometry (GC-MS) system coupled with RSH head space autosampler

 

Gas chromatography–mass spectrometry (GC-MS) is a hybrid analytical technique Where GC separates volatile components in a sample and MS helps fragment the components and identify them on the basis of their mass matching with an inbuilt database. It provides enhanced sample identification, higher sensitivity, an increased range of analyzable samples, and faster results.

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Application

  • Cosmetics –Oils in ointments, creams, lotions, impurities, allergens
  • Food - Fatty acids, esters, aldehydes, alcohols, terpenes
  • Perfumes – Fragrance analysis
  • Essential oil analysis
  • Pharmaceuticals –Impurities in active pharmaceutical ingredients
  • Forensic – Fire debris, poisons and steroids
  • Biological – Narcotics, alcohols, drugs, pollutants
  • Industrial- Inorganic gases, aromatic solvents, in polymer synthesis
  • Solvent trace analysis of food, spices and herbal products

Contact Details: Herbal Technology Section

                                  +94-11-2797322

 

  • Microplate spectrophotometers

SpectraMax® Plus 384 

 Microplate spectrophotometers allow researchers to analyze multiple samples in parallel.

The SpectraMax® Plus 384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 

 

Microplate

Application

  • Endpoint, discontinuous kinetics, or spectral runs in real time
  • DNA quantitation
  • Colorimetric assays
  • ELISAs/EIAs
  • Immunoassays
  • Drug dissolution profiles
  • Enzyme kinetics (e.g., Ki , Km, etc.)
  • Comprehensive data analysis with SoftMax Pro Software

Contact Details: Herbal Technology Section

                                  +94-11-2797322

The SpectraMax® Gemini™ EM

Provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays.

SoftMax® Pro Microplate Data Acquisition and Analysis Software provides convenient data analysis.

The SpectraMax

Application

  • Cell migration assays
  • Live/Dead viability/ cytotoxicity assays
  • Green fluorescent protein
  • NanoOrange protein quantitation
  • PicoGreen DNA detection
  • Molecular beacons
  • Caspase-3
  • Fluorometric protease assays
  • cAMP detection

Contact Details: Herbal Technology Section

                                  +94-11-2797322

 

  • Laborotory Freeze dryer

Freeze dryer executes a low temperature dehydration process typically used to preserve perishable materials, to extend shelf life or make the material more convenient for transport. 

Capacity: 5 L - 8 L

Application

  • Food processing - Vegetables, fruits, tea, coffee etc.
  • Dairy Industry - High value milk proteins, enzymes for probiotics and starter cultures
  • Nutraceuticals – Herbal extracts
  • Starters and Cultures - Yogurts, probiotics

 

Contact Details: Herbal Technology Section

                                  +94-11-2797322

  •  Liquid Chromatography (LC) systems

High performance liquid chromatography (HPLC) systems enable to separate, identify, quantifyand fractionate components in a liquid mixture.

Analytical High Performance Liquid Chromatography (HPLC) system

Liquid Chromatography

Ultra performance liquid chromatography (UPLC) system

Ultra-performance liquid chromatography is a new advanced category of the analytical HPLC with improved chromatographic performance in speed, resolution, and sensitivity.It is a modified HPLC method comprising high pressure and small sized particles (less than 2 µm) used in the column, so the length of the column decreases leading to time saving and reduction in the consumption of solvent. 

 Application

  • Detection and quantification of marker compounds - Medicinal plant extracts/spices
  • Pharmaceutical analysis – Active ingredients, impurities
  • Purity testing of raw materials – Medicinal plant materials
  • Stability evaluation and degradation monitoring of products – Medicinal plant extracts, cosmetics etc.

 

 Semi-preparative High Performance Liquid Chromatography (HPLC) system

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Application

  • Purification of natural product extracts
  • Fractionation and Isolation of single compounds from a compound mixture

 Contact Details: Herbal Technology Section

                                  +94-11-2797322

 

  •  Inverted Research Microscope

Inverted research microscope enables observation of living cells or organisms under more natural conditions by combining automated digital upright & inverted wide field microscopy with multi-mode reading.

Application

Cell culture investigations using live cell imaging, phase contrast, differential interference contrast (DIC), Hoffman modulation contrast, emboss contrast and epi-fluorescence.

Contact Details: Herbal Technology Section

                                  +94-11-2797322

 

 

 

 

 

Last Updated on Monday, 22 February 2021 10:59

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