Research and Development Facilities

Biotechnology Unit at Industrial Technology Institute is a comprehensive well equipped Biotechnology laboratory possessing Molecular Biology, Microbiology, Plant tissue culture and Industrial enzyme fermentation platforms for the interdisciplinary research applications and services of biotechnology.

btu R  D facilitiesMolecular Biology

Molecular Biology 3 copy

The Platform possesses state-of-the-art equipments and expertise to carry out molecular biology based research.

  • Gene cloning, recombinant protein expression in microorganisms,
  • Nucleic acids extraction and purification
  • PCR reaction setup and cleanup
  • On-demand protocol and kits development and validation
  • Plant species authentication through DNA barcoding
  • Identification of microorganisms through DNA sequencing
  • Genetic engineering of bacteria and plants
  • Agarose gel electrophoresis
  • Analysis of protein by sodium dodecyl sulfate gel electrophoresis (SDS-PAGE)

 

Microbiology

Microbiology 3 copy

The platform is devoted to the handling the microorganisms used in Biotechnology, analysis of the diversity and the exploitation of microorganisms. This platform offers facilities and instruments for the culture of microorganisms.

  • Culturing and sub-culturing of bacteria and fungus
  • Isolation of bacteria for Bioremediation
  • Determination of antimicrobial properties of compounds
  • Preparation of competent cells and bacterial transformation
  • Identification of microorganisms through DNA sequencing

 

Tissue Culture

Tissue Culture 3 copy

We have a well equipped state of the art production facility for the research and production of the tissue cultured planting material. Our laboratory facilities for plant tissue culture include the following:

  • A general washing area
  • A media preparation, sterilization, and storage area
  • An aseptic transfer area
  • Environmentally controlled culture rooms

Tissue culture, also known as micropropagation, is a propagation method used to produce plants under sterile conditions. This method uses plant explants (plant parts) or seeds that have been sterilized before being placed in containers with a growing medium that has some nutrients added. The explants or seeds, the containers and the medium have all been sterilized, and prevent any cut or torn tissue, or the entire explant or seed itself, from becoming infected with a microorganism of some kind and rotting during the time these plant parts require to become rooted or to multiply. In our lab, we are mainly involving in rice tissue culture and successfully developed tissue culture protocols for popular rice varieties as well as for traditional rice varieties available in the Sri Lanka. We can offer you a wide range of tropical and temperate tissue cultured plants with an incomparable quality. For any further information regarding our products, do not hesitate to contact us.

 

 

Industrial fermentation

Fermentation is a method of generating enzymes for industrial purposes. Fermentation involves the use of micro organisms, like bacteria and yeast to produce the enzymes. Biotechnology Unit has undertaken an ambitious project to produce common industrial enzymes such as α-amylase, cellulases and lipases for selected industries by fermentation.

 

Instrumentation

The Biotechnology Unit at Industrial Technology Institute possesses state-of-the-art equipments for biotechnology research and services. Instrumentation used at the biotechnology unit include following.
btu22

  • PCR thermal cycler (gradient)
  • Eporator (Electroporation apparatus)
  • Horizontal Gel Electrophoresis unit with Molecular Imager Gel Doc and gel documentation system
  • Incubators
  • pH meter
  • Refrigerated Centrifuge
  • Horizontal Laminar Flow
  • Autoclave machine

 

 

 

Last Updated on Friday, 07 February 2014 14:34

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